Regulatory

Part:BBa_K4388013:Design

Designed by: Venkata Kalyan Ghadiyaram   Group: iGEM22_KCL_UK   (2022-10-11)


Strong BBa_J231000 promoter fused to lac operator and strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NheI site removed from BBa_J231000 sequence.


Source

Fusion of the lac promoter+BBa_J23100 alongside the lac promoter

References